The Harris laboratory currently examines the microcirculation in the pathology associated with diabetic retinopathy and inflammatory bowel disease. In both diseases, changes occur in microvascular flow, hypoxia, and permeability, with the mechanisms and consequences of these changes still under investigation.
Intravital microscopy (brightfield and fluorescence)
Measurement of microvascular flow and permeability
Immunostaining of tissue sections
Computerized video analysis of microscope images
Top: Texas Red albumin (red dye) flowing through vessels of the mouse retina; cell nuclei are stained blue with DAPI.
Middle: Pimonidazole (brown) staining showing areas of hypoxia in the mouse proximal colon; cell nuclei are stained blue with hematoxylin.
Bottom: Green staining of the vessels (PECAM-1, specifically) in the mouse proximal colon; cell nuclei are stained with propidium iodide.